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The relief got the colony through the winter, but the supplies expected in the spring had not arrived by September. De Luna ordered the remainder of his force to march to the large native town of Coca, but the men mutinied. Bloodshed was averted by the settlement's missionaries.
Meanwhile, Spanish officials had issued orders recalling de Luna on [[January 30]], [[1561]]]. Soon after, [[Wikipedia:Ángel de Villafañe|Ángel de Villafañe]] arrived in Pensacola Bay on [[March 9]] and offered to take all who wished to leave on an expedition to [[Wikipedia:Cuba|Cuba]] and Santa Elena. De Luna relented and agreed to leave. The Pensacola colony was inhabited for several more months by Captain Biedma and a detachment of fifty men who Villafañe had left there, in case further orders arrived from Viceroy Velasco.
When they sailed away, the area was not populated again by Europeans until [[1698]], when [[First Spanish period|Spanish]] forces under [[Andrés de Arriola]] established the [[Presidio de Santa María de Galve]].